Expression and production optimization of the cationic antimicrobial peptide - indolicidin by the recombinant E. coli C41 (DE3) clones
نویسندگان
چکیده
منابع مشابه
TRANSFORMATION OF ESCHERICHIA COLI WITH pET21A + AND EXPRESSION OF THE NOVEL ANTIMICROBIAL PEPTIDE INDOLICIDIN
Indolicidin is an antimicrobial cationic peptide naturally present in the bovine neutrophils. In this study, transformed Escherichia coli cells were employed for greater and cost effective production of indolicidin. Four strains of E. coli [C41 (DE3), C43 (DE3), C41 (DE3) pLysS & C43 (DE3) pLysS] were transformed with recombinant plasmid pET21A carrying the indolicidin gene and were successfull...
متن کاملThe toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3).
Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system. Even when the toxicity of the plasmids is so high that it prevents transformation in the strain BL21(DE3), the to...
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Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
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Background: Antimicrobial peptides have been considered as the key to change in the current antibiotics. AcAMP, Antimicrobial peptide is one of them, which has been purified from the Aspergillus clavatus ES1 fungus, and its biochemical properties and characterization indicate that this peptide has unique properties. The aim of this study was to express the gene of AcAMP peptide in Escherichia c...
متن کاملOptimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
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ژورنال
عنوان ژورنال: Acta Biologica Szegediensis
سال: 2018
ISSN: 1588-4082
DOI: 10.14232/abs.2018.1.61-66